Biotin is an unnumbered member of the B-complex family, normally only required in minute amounts. Biotin, a water-soluble vitamin, is used as co-factor of enzymes involved in fatty acid metabolism, gluco-neogenesis, and amino acid catabolism, making biotin essential in maintaining metabolic homeostasis. Biotin plays an important role in metabolic functioning as a coenzyme carrier of activated carbon dioxide in the TCA cycle (also known as the Krebs cycle). n its coenzyme form, biotin synthesizes glucose from non-carbohydrate sources, and synthesizes and breaks down certain fatty acids and amino acids